获奖作品基本信息
| 年份 | 2019 |
| 学科 | 生物化学 Biochemistry |
| 国家/州 | CT，United States of America |
获奖作品名称
Deuterium Oxide (D2O) on Maintaining Viability in Coliphage Bacteriophages under Low Temperatures to Model Live Attenuated Viral Vaccine Additives
获奖作品摘要
Viral particles used in vaccines and viral therapies are often damaged due to the molecular movement of their storage solution and require storage at low temperatures to reduce the velocity of the water molecules. Deuterium oxide (D2O) is made with an isotope of hydrogen that increases the density of water to 1.11 g/mL; when viral particles are stored in D2O the increased weight reduces the molecular speed of the solution, reducing trauma to the particles, increasing the temperature in which samples can be stored. A T4 bacteriophage was used to test how a viral particle would react to its environment and deteriorate over time while stored in D2O and deionized water. A sample of Coliphage bacteriophages stored in D2O was compared to a sample stored in deionized water at 16℃ to determine the infectivity titer of the samples over time using plaque assays. The sample stored in D2O showed significantly less deterioration over time and slowed the rate of degradation to 6% that of deionized water. D2O proved to be a more advantageous solution than deionized water in supporting the health of the phages and is a promising storage additive for viral samples. This solution has application for use to increase the storage temperature of live attenuated viral vaccines, such as the Ebola vaccine, rVSV EBOV that often require storage at very low temperatures during transport and storage to remain effective and viable for administration to patients.
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ISEF 简介
英特尔国际科学与工程大奖赛，简称 "ISEF"，由美国 Society for Science and the Public（科学和公共服务协会）主办，英特尔公司冠名赞助，是全球规模最大、等级最高的中学生的科研科创赛事。ISEF 的竞赛学科包括了所有数学、自然科学、工程的全部领域和部分社会科学。ISEF 素有全球青少年科学竞赛的“世界杯”之美誉，旨在鼓励学生团队协作，开拓创新，长期专一深入地研究自己感兴趣的课题。
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学科简介：生物化学 Biochemistry
The study of the chemical basis of processes occurring in living organisms, including the processes by which these substances enter into, or are formed in, the organisms and react with each other and the environment.
Subcategories:
Analytical Biochemistry (ANB): The study of biochemical components found in a cell or other biological sample. The study of the separation, identification, and quantification of chemical components relevant to living organisms.
General Biochemistry (GNR): The study of chemical and physiochemical processes, including interactions and reactions, relevant to living organisms.
Medicinal Biochemistry (MED): The study of biochemical processes within the human body, with special reference to health and disease.
Structural Biochemistry (STR): The study of components, functions, and structures of molecules of cells within living organisms. Projects in this subcategory can address various variables of cells through the theories of Chemistry and the laws of Physics.
Other (OTH): Studies that cannot be assigned to one of the above subcategories. If the project involves multiple subcategories, the principal subcategory should be chosen instead of Other.
