获奖作品基本信息
| 年份 | 2019 |
| 学科 | 微生物学 MICROBIOLOGY |
| 国家/州 | NY，United States of America |
获奖作品名称
Investigating the Role of the Novel ESCRT-III Recruiter CCDC11 in HIV Viral Budding: Identifying a Potential Target for Antiviral Therapy
获奖作品摘要
Acquired immunodeficiency syndrome (AIDS) is a life-threatening condition caused by infection of the human immunodeficiency virus (HIV). Compromising the immune system, HIV reduces the body's ability to fight the organisms that cause disease. In an effort to discover potential targets for antiviral therapies, we explored the role of coiled-coil domain-containing 11 (CCDC11) in HIV-1 budding. CCDC11 plays an important role in ciliogenesis and cytokinesis. During cytokinesis, it recruits the endosomal sorting complex required for transport III (ESCRT-III) membrane scission complex to the midbody to mediate the physical separation of two dividing daughter cells. The ESCRT-III machinery is also integral to the viral budding process of HIV-1 and many other viruses. Therefore, we hypothesized that CCDC11 is also required for viral budding. To investigate this possibility, we established CCDC11-knockout human embryonic kidney (HEK) 293T cells using the CRISPR-Cas9 technology. Indels of the CCDC11 gene were confirmed by DNA sequencing, and protein levels were assessed via western blotting and immunofluorescence staining. To determine the effect of CCDC11-knockout on HIV-1 budding, we employed the Enzyme-linked immunosorbent assay (ELISA) p24 capture assay to indirectly assess viral release by measuring the relative concentration of HIV-1 Gag structural protein (p24) or mutant P7L-Gag in cell media. Our results demonstrate that ectopic overexpression of CCDC11 markedly enhances whereas depletion of CCDC11 in the knockout cells dramatically reduces viral particle release. The defective viral budding in CCDC11-knockout cells was restored when wild-type CCDC11 was re-expressed. Collectively, our data suggest that CCDC11 is critical for efficient HIV-1 budding.
高中生科研 英特尔 Intel ISEF
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高中生科研竞赛 英特尔 Intel ISEF 简介
英特尔国际科学与工程大奖赛，简称 "ISEF"，由美国 Society for Science and the Public（科学和公共服务协会）主办，英特尔公司冠名赞助，是全球规模最大、等级最高的科研科创赛事。ISEF 的竞赛学科包括了所有数学、自然科学、工程的全部领域和部分社会科学。ISEF 素有全球青少年科学竞赛的“世界杯”之美誉，旨在鼓励学生团队协作，开拓创新，长期专一深入地研究自己感兴趣的课题。
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学科简介：微生物学 MICROBIOLOGY
The study of micro-organisms, including bacteria, viruses, fungi, prokaryotes, and simple eukaryotes as well as antimicrobial and antibiotic substances.
Subcategories:
Antimicrobials and Antibiotics (ANT): Studies involving substances that kill or inhibit the growth of a microorganism.
Applied Microbiology (APL): The study of microorganisms having potential applications in human, animal or plant health or the use of microorganisms in the production of energy.
Bacteriology (BAC): The study of bacteria and bacterial diseases and the microorganisms responsible for causing a disease. This field focuses on ideas spanning a wide range of topics, from identification and characterization of bacteria, all the way to the development of effective vacancies to combat various types of bacteria.
Environmental Microbiology (ENV): Studies involving microbial interactions and processes within the environment. Air microbiology, soil microbiology and water microbiology as well as the study of biofilms would be included in this subcategory.
Microbial Genetics (GEN): The study of the genetics of microorganisms such as bacteria, archaea and some protozoa and fungi and their chromosomes, plasmids, transposons and phages. Studies can also include gene transfer systems such as transformation, conjugation and transduction.
Virology (VIR): The study of viruses – submicroscopic, parasitic particles of genetic material contained in a protein coat – and virus-like agents. Research in this subcategory may focus on the development and effectiveness of treatments for viruses, the development and life cycle of a particular virus, or how the immune system recognizes a virus and what stimulates immune responses.
Other (OTH): Studies that cannot be assigned to one of the above subcategories. If the project involves multiple subcategories, the principal subcategory should be chosen instead of Other.
