|学校||Roanoke Valley Governor's School for Science and Technology|
|国家/州||VA，United States of America|
Novel Use of Phenolic Compounds for Epstein-Barr Virus Lytic Induction
Epstein-Barr virus is an oncogenic herpesvirus which causes over 150,000 fatalities annually and has no FDA-approved treatment. Latent EBV is characterized by limited gene expression, which allows it to evade immune detection. Artificially inducing lytic replication increases gene expression and allows targeted treatment of EBV seropositive tumors. This research evaluated the efficacy of two natural polyphenolic compounds in terms of I) their ability to inhibit primary EBV infection and II) their effect on latently infected Burkitt’s lymphoma cells. In Phase I, EBV was conjugated with FITC, allowing for visualization of infection. Ramos cells were assessed at three time-points: 24, 48, and 72 hours. EBV infection was apparent when assessed via microscopy. Both resveratrol and corilagin upregulated BZLF1 expression and increased viral load. Thus, primary EBV infection was not inhibited. In Phase II, Raji, an EBV+ cell line, was treated with either phenolic compounds or 3mM sodium butyrate. RT-qPCR for lytic genes BZLF1 and BALF5 concluded that resveratrol enhanced the expression of these genes, while corilagin inhibited it. Increased viral load was seen in resveratrol treated groups, due to the upregulation of these lytic genes. Overall, resveratrol and sodium butyrate mediated lytic induction provides a potential future treatment combination to be evaluated in the therapy of EBV seropositive tumors. Additionally, inhibitory effects of corilagin in lytic EBV infections mimic nucleosides, which have been previously shown to inhibit both EBV and cellular proliferation.
高中生科研 英特尔 Intel ISEF
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高中生科研竞赛 英特尔 Intel ISEF 简介
英特尔国际科学与工程大奖赛，简称 "ISEF"，由美国 Society for Science and the Public（科学和公共服务协会）主办，英特尔公司冠名赞助，是全球规模最大、等级最高的中学生的科研科创赛事。ISEF 的竞赛学科包括了所有数学、自然科学、工程的全部领域和部分社会科学。ISEF 素有全球青少年科学竞赛的“世界杯”之美誉，旨在鼓励学生团队协作，开拓创新，长期专一深入地研究自己感兴趣的课题。
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The study of micro-organisms, including bacteria, viruses, fungi, prokaryotes, and simple eukaryotes as well as antimicrobial and antibiotic substances.
Antimicrobials and Antibiotics (ANT): Studies involving substances that kill or inhibit the growth of a microorganism.
Applied Microbiology (APL): The study of microorganisms having potential applications in human, animal or plant health or the use of microorganisms in the production of energy.
Bacteriology (BAC): The study of bacteria and bacterial diseases and the microorganisms responsible for causing a disease. This field focuses on ideas spanning a wide range of topics, from identification and characterization of bacteria, all the way to the development of effective vacancies to combat various types of bacteria.
Environmental Microbiology (ENV): Studies involving microbial interactions and processes within the environment. Air microbiology, soil microbiology and water microbiology as well as the study of biofilms would be included in this subcategory.
Microbial Genetics (GEN): The study of the genetics of microorganisms such as bacteria, archaea and some protozoa and fungi and their chromosomes, plasmids, transposons and phages. Studies can also include gene transfer systems such as transformation, conjugation and transduction.
Virology (VIR): The study of viruses – submicroscopic, parasitic particles of genetic material contained in a protein coat – and virus-like agents. Research in this subcategory may focus on the development and effectiveness of treatments for viruses, the development and life cycle of a particular virus, or how the immune system recognizes a virus and what stimulates immune responses.
Other (OTH): Studies that cannot be assigned to one of the above subcategories. If the project involves multiple subcategories, the principal subcategory should be chosen instead of Other.